Fig. 1

Trehalose accumulates under high-temperature stress. The changes in intracellular metabolites in P. ostreatus mycelia treated with heat (40 °C) for 0–48 h were detected by LC–MS, and the intermediate metabolites in trehalose metabolism showed different changes. The changes in the contents of different metabolites under high-temperature stress are indicated in the graph. The abscissa of the graph shows the treatment time, among which 48 + 6 h means treatment with heat for 48 h and then recovery for 6 h at 28 °C. The ordinate of the graph shows normalized peak area data, which represent the relative contents of various metabolites. The data used to construct the graph are the average values of seven biological repeats and are expressed as mean ± SEM values. TPS1: trehalose-6-phosphate synthase 1, TPS2: trehalose-6-phosphate synthase 2, G-6-P: glucose 6-phosphate, T-6-P: trehalose 6-phosphate. The significance analysis was first conducted within 28 °C or 40 °C group separately, and the significant differences were marked with different lowercase letters over the columns (P < 0.05 according to the Tukey or Kruskal-Wallis test); and then conducted between 28 and 40 °C whole groups, the significant difference were indicated by *, *P < 0.05 according to the Bonferroni test, ns means no significance. The lowercase letters between 28 and 40 °C groups have no comparability in significance analysis