Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol

Table 2 Efficiency parameters of different cell catalysts (LmSP_pQE30) preparations in GG synthesis

Treatment	GG yield		Catalyst activity^a	GG concentration	Space–time yield^b	Reaction^c/site^d selectivity
Treatment	2 h (%)	24 h (%)	U/g_CDM	g/L	g/L/h	%
Non-permeabilized	0	44	11.5	32.6	1.4	93/88
Extracted	65	86	846	64.0	12.1	89/88
Freeze-thawed	45	86	598	64.3	7.6	92/88
Freeze-dried	42	80	553	61.7	7.5	87/88

^aOne U is the enzyme amount producing 1 µmol GG/min
^bCalculated for a sucrose conversion of 95%, except for non-permeabilized cells, where conversion was 50%
^c,dPercent ratio of ^c GG formed/Fru released (= sucrose converted) and ^d GG/(1-GG + GG)

ISSN: 1475-2859