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Table 3 Kinetic parameters of BcGalB

From: Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Substrate

KM (mM)

kcat (s−1)

kcat/KM (mM−1 s−1)

ONP-β-gal

D-lactose

0.723

136.2

5466.7

1603.7

756.2

11.8

  1. KM, kcat and kcat/KM values towards the natural and artificial substrates are reported. Standard deviations were lower than 2% of the calculated values
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Microbial Cell Factories

ISSN: 1475-2859

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