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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance

Fig. 1

Overexpression of Vel1 gene in T. asperellum GDFS1009. a Schematic diagram of the constructed plasmid pCAMBIA1300 -Vel1. b PCR amplification of TrpC promoter, TrpC terminator and Vel1 ORF (1: TrpC promoter; 2: TrpC terminator; 3: Vel1 ORF; M DS 2000 marker) c PCR results for transformant identification (M: 1 KB marker; 1: Vel1 ORF; 2: over expression cassette containing TrpC promoter, Vel1 ORF and TrpC terminator). d The phenotypes of Wild type (TA) and e phenotype of Vel1 gene overexpress transformants grown on PDA plates

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