Fig. 4
DAP production with EcLDCc-L6KD variants. Experimental conditions: Purified CatIBs from cells with an OD600 nm = 12.5, 10 mM l-lysine, 0.1 mM PLP in 50 mM Kpi buffer (pH 7.2). Sampling times: 0, 3, 6, 12 and 20 min. nB=3, except 3 min SG-L6KD (nB=2), and nA=3 (not visible, due to low standard deviation). Conversion calculated based on DAP formation from l-lysine measured by HPLC