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Fig. 5 | Microbial Cell Factories

Fig. 5

From: An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system

Fig. 5

Carbon sources and buffers affect T3SS transcriptional activity. Transcriptional activity at the inv, prg, and sic loci for no added carbon source (“none”), glucose, and glycerol in the media listed in Additional file 1: Table S7 was measured over time by performing flow cytometry on ASTE13 strains containing invE:gfpmut2, prgH:gfpmut2, or sipC:gfpmut2 transcriptional fusions. Error bars represent standard error of the GFP geometric mean (“Mean Fluorescence”) for three biological replicates

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