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Fig. 2 | Microbial Cell Factories

Fig. 2

From: An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system

Fig. 2

Carbon sources decrease secretion titer in LB-L. a Relative bulk secretion titer and b expression of SptP-DH-2xFLAG-6xHis. Expression and secretion titer were normalized to LB-L with no carbon source using densitometry measurements on western blots. b Transcriptional activity at the inv, prg, and sic loci was measured by performing flow cytometry on strains with transcriptional fusions of GFPmut2 at the appropriate locus. All error bars represent standard error for three biological replicates

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Microbial Cell Factories

ISSN: 1475-2859

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