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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor

Fig. 1

SDS-PAGE gels of cell extracts prepared from various recombinant P. pastoris (a) expressing XR alone or (b) co-expressing XR with GDH. (a) Lanes 1 to 5: pPIC4Kx-PsXYL1 clones 1000–1, 1000–2, 4000–1, 4000–2, and 4000–3, respectively. Lanes 6–10: pPIC4Kx-NcXR clones 1000–1, 1000–3, 4000–2, 4000–3, and 4000–6, respectively. Lanes 11–15: pPIC4Kx-CpXr clones 4000–8, 4000–6, 4000–2, 1000–6, and 1000–2, respectively. (b) Lanes 1 to 5: pPIC4Kx + PsXLY1 + gdh clones 1000–7, 1000–8, 4000–4, 4000–7, and 4000–8, respectively. Lanes 6 to 10: pPIC4Kx + NcXR + gdh clones 1000–1, 1000–2, 4000–1, 4000–2, and 4000–3, respectively. Lanes 11 to 15: pPIC4Kx + CpXR + gdh clones 1000–5, 1000–6, 4000–4, 4000–5, and 4000–6, respectively. Lane C contained cell extracts of P. pastoris GS115 transformed with an empty pPIC4Kx. The red arrows indicated the over-expressed XR proteins

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Microbial Cell Factories

ISSN: 1475-2859

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