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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Title: insoluble proteins catch heterologous soluble proteins into inclusion bodies by intermolecular interaction of aggregating peptides

Fig. 1

Effect of the addition of L6K2 in the aggregation propensity of fluorescent proteins. a Schematic representation of the recombinant genes is shown displaying protein domains by separate squares. H6 indicates the presence of a Hisx6 tag. PT corresponds to a linker with the indicated amino acid sequence. b Comparative aggregation propensity of iRFP and GFP in the presence of L6K2. VP1GFPH6 corresponds to a GFP fused to VP1 considered as positive control of the experiment. c Relative solubility (%) of recombinant proteins detailed in panel a analyzed by Western Blotting. Equivalent number of transformed ClearColi cells were lysed and soluble and insoluble cells fractions were separated. All proteins were detected with anti-his antibody

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Microbial Cell Factories

ISSN: 1475-2859

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