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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Multiple copies of the oxytetracycline gene cluster in selected Streptomyces rimosus strains can provide significantly increased titers

Fig. 4

Pulse-field gel electrophoresis (PFGE; left) and Southern blotting (right) of S. rimosus M4018 transformants (as indicated) carrying the chromosomally integrated pYAC-ΦC31-Ts-OTC plasmid. a Genomic DNA digested with the XhoI restriction enzyme. b Genomic DNA digested with the AseI restriction enzyme. The Southern blotting (right) of the PFGE gels (left) was through hybridization with probes generated from external homologies of pYAC ΦC31-Ts-h. L, Lambda concatamers, C, S. cerevisiae chromosomes; M, S. rimosus M4018 parental strain; O4, S. rimosus M4018 transformant with pYAC-ΦC31-Ts-h (control); O24-O97, selected S. rimosus M4018::otc transformants. Arrows indicate presence of the otc cluster

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Microbial Cell Factories

ISSN: 1475-2859

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