Fig. 5From: Direct RBS Engineering of the biosynthetic gene cluster for efficient productivity of violaceins in E. coliThe fermentation titers of the strains in the third and fourth round of RBS mutagenesis and (BCDE)m optimized by one-factor-at-one-time method. aĀ The titers of (BCD)m, (BCE)m, (BDE)m, (CDE)m and (BCDE)m compared with the wild type strain E. coli BL21(DE3) (Vio12472). bĀ The fermentation titers of (BCDE)m at different temperatures (16 ā, 20 ā, 25 ā, 30 ā, 37 ā). cĀ The fermentation titers of (BCDE)m at various concentrations of IPTG (0.01, 0.02, 0.04, 0.06, 0.08, 0.10, 0.20, 0.40, 0.60, 0.80 and 1.00Ā mM). (D) The fermentation titers for violacein and deoxyviolacein produced by (BCDE)m in the 192 hās cultivation periodBack to article page