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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Tunable expression rate control of a growth-decoupled T7 expression system by l-arabinose only

Fig. 6

SDS-Page analysis of end samples (1 mg) from fed-batch like cultivation of E. coli strain BL21-AI (a, b) and BL21-AI<gp2> (c, d) expressing GFPmut3.1, showing distribution of soluble (S) and insoluble (IB) GFP. Lane M, molecular weight marker (NovexMark12 Unstained Standard); Induction was performed with (a, c) Lane 2: non-induced; Lane 3/4: 100 mM l-arabinose + 1 mM IPTG; Lane 5/6: 100 mM l-arabinose; Lane 7/8: 50 mM l-arabinose; Lane 9/10: 25 mM l-arabinose; Lane 11/12: 5 mM l-arabinose; (b, d) Lane 2: non-induced; Lane 3/4: 1 mM L-arabinose; Lane 5/6: 0.25 mM l-arabinose; Lane 7/8: 0.025 mM L-arabinose

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Microbial Cell Factories

ISSN: 1475-2859

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