Table 3 The primers used for the construction of the fusion enzyme YsADH-(linker)-TkNOX, YsADH-(GSG)-TkNOX-(GSG)-VsHGB or VsHGB-(GSG)-TkNOX-(GSG)-YsADH
Linker | Gene | Primers | |
|---|---|---|---|
GGGGS | ADH | F | 5′-TAACTTTAATAAGGAGATATACCATGGGCATGTCTATTATAAAAAGCTATGCC-3′ |
R | 5′-ACCACGGTTTTACGTTCCATGCTGCCGCCGCCGCCAAAGTCGGCTTGCAGTACCAC-3′ | ||
TkNOX | F | 5′-TGGTACTGCAAGCCGACTTTGGCGGCGGCGGCAGCATGGAACGTAAAACCGTGGTG-3′ | |
R | 5′-ACTTAAGCATTATGCGGCCGCAAGCTTTCAAAATTTCAGAACACGTGCC-3′ | ||
GSG | ADH | F | 5′-TAACTTTAATAAGGAGATATACCATGGGCATGTCTATTATAAAAAGCTATGCC-3′ |
R | 5′-CACCACGGTTTTACGTTCCATGCCGCTGCCAAAGTCGGCTTGCAGTACCAC-3′ | ||
TkNOX | F | 5′-GTGGTACTGCAAGCCGACTTTGGCAGCGGCATGGAACGTAAAACCGTGGTG-3′ | |
R | 5′-ACTTAAGCATTATGCGGCCGCAAGCTTTCAAAATTTCAGAACACGTGCC-3′ | ||
(GGGGS)2 | ADH | F | 5′-TAACTTTAATAAGGAGATATACCATGGGCATGTCTATTATAAAAAGCTATGCC-3′ |
R | 5′-CACCACGGTTTTACGTTCCATGCTGCCGCCGCCGCCGCTGCCGCCGCCGCCAAAGTCGGCTTGCAGTACCAC-3′ | ||
TkNOX | F | 5′-GTGGTACTGCAAGCCGACTTTGGCGGCGGCGGCAGCGGCGGCGGCGGCAGCATGGAACGTAAAACCGTGGTG-3′ | |
R | 5′-ACTTAAGCATTATGCGGCCGCAAGCTTTCAAAATTTCAGAACACGTGCC-3′ | ||
(GSG)2 | ADH | F | 5′-TAACTTTAATAAGGAGATATACCATGGGCATGTCTATTATAAAAAGCTATGCC-3′ |
R | 5′-CACCACGGTTTTACGTTCCATGCCGCTGCCGCCGCTGCCAAAGTCGGCTTGCAGTACCAC-3′ | ||
TkNOX | F | 5′-GTGGTACTGCAAGCCGACTTTGGCAGCGGCGGCAGCGGCATGGAACGTAAAACCGTGGTG-3′ | |
R | 5′-ACTTAAGCATTATGCGGCCGCAAGCTTTCAAAATTTCAGAACACGTGCC-3′ | ||
GSG | YsADH-(GSG)-TkNOX | F | 5′-TAACTTTAATAAGGAGATATACCATGGGCATGTCTATTATAAAAAGCTATGCC-3′ |
R | 5′-GGTCTGCTGGTCCAGCATGCCGCTGCCAAATTTCAGAACACGTGCC-3′ | ||
VsHGB | F | 5′-GGCACGTGTTCTGAAATTTGGCAGCGGCATGCTGGACCAGCAGACC-3′ | |
R | 5′-ACTTAAGCATTATGCGGCCGCAAGCTTTTATTCAACTGCCTGAGCG-3′ | ||
GSG | VsHGB | F | 5′-TAACTTTAATAAGGAGATATACCATGGATGCTGGACCAGCAGACC-3′ |
R | 5′-ATAACCACCACGGTTTTACGTTCCATGCCGCTGCCTTCAACTGCCTGAGCG-3′ | ||
TkNOX | F | 5-’ATCTGTACGCTCAGGCAGTTGAAGGCAGCGGCATGGAACGTAAAACCGTGG-3′ | |
R | 5′-CGGCATAGCTTTTTATAATAGACATGCGCCGCTGCCAAATTTCAGAACACGTGCC-3′ | ||
YsADH | F | 5′-GGCACGTGTTCTGAAATTTGGCAGCGGCGCATGTCTATTATAAAAAGCTATGCCG-3′ | |
R | 5′-ACTTAAGCATTATGCGGCCGC AAGCTTTCAAAAGTCGGCTTGCAG-3′ |