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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 1

SDS-PAGE analysis of YsADH, TkNOX and/or VsHGB in the cell-free extracts. Lane M, marker; lane 1, expression of VsHGB (15.5 kDa) alone; lane 2, expression of YsADH (33.2 kDa) alone; lane 3, coexpression of YsADH (33.2 kDa) and TkNOX (43.4 kDa); lane 4, coexpression of YsADH (33.2 kDa), TkNOX (43.4 kDa) and VsHGB (15.5 kDa); lane 5, no induction of cells coexpressing YsADH, TkNOX or VsHGB as the control. The percentage of acrylamide in the resolving gel was 12%. The value in the bracket represents the apparent molecular mass of YsADH, TkNOX or VsHGB

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