Engineering Escherichia coli for the utilization of ethylene glycol

Table 1 Yield and orthogonality metrics for chemical production from different substrates

	Succinate		Ethanol		Glycolate		2,3-Butanediol
	Score	Yield	Score	Yield	Score	Yield	Score	Yield
Ethylene glycol	0.54	0.95	0.61	0.62	0.67	1.22	0.66	0.66
Formate	0.47	0.29	0.5	0.14	0.48	0.33	0.49	0.18
Glucose	0.41	1.12	0.44	0.51	0.41	0.85	0.47	0.50
Xylose	0.36	1.12	0.36	0.51	0.34	1.01	0.4	0.50

The orthogonality scores for various products are shown comparing two substrates that can be generated electrochemically (EG and formate) against conventional sugar substrates (glucose and xylose) assimilated via their natural pathways. Formate has orthogonality scores similar to many sugar consuming pathways, indicating its utilization is relatively complex and interconnected with native growth. Ethylene glycol (EG) exhibits the highest orthogonality scores, and has higher or comparable theoretical yields relative to the other substrates. Yield is given as g of product per 1 g of substrate

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