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Fig. 5 | Microbial Cell Factories

Fig. 5

From: High copy number and highly stable Escherichia coliBacillus subtilis shuttle plasmids based on pWB980

Fig. 5

Alkaline protease productions in B. subtilis WB600. a Protease activities in supernatants were determined during Spro1-expressions with different plasmids in B. subtilis WB600. The highest activity was obtained by pUC980-2 with 4818 U/mL. b SDS-PAGE analysis of Spro1 in culture supernatants. Lane M was protein molecular weight marker. The Spro1 protein was 26.7 kDa as shown in the frame

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