Fig. 5From: High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980Alkaline protease productions in B. subtilis WB600. a Protease activities in supernatants were determined during Spro1-expressions with different plasmids in B. subtilis WB600. The highest activity was obtained by pUC980-2 with 4818 U/mL. b SDS-PAGE analysis of Spro1 in culture supernatants. Lane M was protein molecular weight marker. The Spro1 protein was 26.7 kDa as shown in the frameBack to article page