Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of l-lysine production from mixed sugar

Table 3 Comparison of intracellular nucleotides concentrations in original strain and the genetically defined C. glutamicum strains (μmol/(g DCW))

Strains	NADH	NAD⁺	NADH/NAD⁺	NADPH	NADP⁺	NADPH/NADP⁺	ATP
K-1	1.69 ± 0.22	7.68 ± 0.83	0.22 ± 0.03	1.71 ± 0.12	1.47 ± 0.15	1.16 ± 0.10	4.98 ± 0.42
K-2	1.67 ± 0.23	7.59 ± 0.66	0.22 ± 0.01	1.74 ± 0.21	1.42 ± 0.19	1.23 ± 0.13	4.87 ± 0.53
K-3	0.28 ± 0.02	2.02 ± 0.24	0.14 ± 0.02	0.25 ± 0.04	0.30 ± 007	0.82 ± 0.09	0.98 ± 0.13
K-4	1.03 ± 0.15	5.42 ± 0.78	0.19 ± 0.03	0.99 ± 0.17	0.94 ± 0.12	1.05 ± 0.14	2.68 ± 0.19
K-5	1.51 ± 0.18	7.57 ± 0.81	0.20 ± 0.01	1.57 ± 0.11	1.38 ± 0.15	1.14 ± 0.13	2.92 ± 0.35
K-6	1.54 ± 0.22	7.69 ± 0.83	0.20 ± 0.02	1.48 ± 0.18	1.31 ± 0.16	1.13 ± 0.10	3.08 ± 0.32
K-7	0.93 ± 0.23	8.58 ± 0.67	0.11 ± 0.03	1.41 ± 0.12	1.25 ± 0.13	1.13 ± 0.21	3.43 ± 0.45
K-8	0.98 ± 0.22	8.91 ± 0.79	0.11 ± 0.01	1.16 ± 0.14	1.05 ± 0.10	1.10 ± 0.08	4.69 ± 0.47

Exponentially growing cells cultured in CgXII^IP with 40 g/L glucose as sole carbon source in shake flasks were used for analysis
All data are meaning values of three determinations of three independent experiments with ± SD

ISSN: 1475-2859