Skip to main content

Table 1 DCW, Cell growth rate, l-lysine production, and substrate consumption rate of original strain C. glutamicum K-1 and its derivatives in shake flask experiments

From: Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of l-lysine production from mixed sugar

Strains

Sugar

Glucose

Fructose

Sucrose

Molasses

DCW

μmax.

PLys

qs

DCW

μmax.

PLys

qs

DCW

μmax.

PLys

qs

DCW

μmax.

PLys

q as

K-1

10.9 ± 1.2

0.25

24.1 ± 1.6

6.26 ± 0.57

8.6 ± 0.5

0.2

17.2 ± 1.8

5.13 ± 0.40

9.9 ± 0.8

0.24

18.9 ± 1.2

5.18 ± 0.32

10.3 ± 1.2

0.2

15.8 ± 2.1

4.38 ± 0.47

K-2

10.6 ± 0.5

0.25

24.3 ± 2.9

6.25 ± 0.66

8.9 ± 0.5

0.21

18.5 ± 1.7

6.06 ± 0.37

10.1 ± 0.6

0.24

23.9 ± 1.6

8.07 ± 0.51

9.8 ± 0.7

0.23

21.3 ± 2.1

7.74 ± 0.45

K-3

1.8 ± 0.2

0.06

0.5 ± 0.06

1.02 ± 0.13

2.1 ± 0.2

0.07

1.3 ± 0.3

1.21 ± 0.05

9.9 ± 1.0

0.23

24.2 ± 1.3

8.06 ± 0.54

9.8 ± 0.3

0.23

22.0 ± 1.8

7.80 ± 0.56

K-4

5.3 ± 0.7

0.12

10.4 ± 1.5

3.38 ± 0.54

9.7 ± 0.6

0.22

23.5 ± 2.2

8.05 ± 0.31

9.8 ± 1.3

0.23

24.1 ± 2.2

8.10 ± 0.72

10.0 ± 1.5

0.24

22.5 ± 1.9

7.95 ± 0.52

K-5

8.9 ± 1.0

0.18

25.3 ± 2.2

7.89 ± 0.71

10.0 ± 1.3

0.22

23.8 ± 3.0

8.06 ± 0.74

9.7 ± 0.9

0.22

24.7 ± 2.7

8.12 ± 0.53

9.8 ± 1.0

0.22

22.8 ± 2.5

7.99 ± 0.63

K-6

10.1 ± 1.4

0.23

26.0 ± 3.0

8.73 ± 0.48

10.2 ± 0.8

0.23

24.9 ± 2.5

8.21 ± 0.57

10.0 ± 1.0

0.23

25.4 ± 1.8

8.33 ± 0.75

9.8 ± 0.6

0.22

23.5 ± 1.5

8.16 ± 0.48

K-7

11.4 ± 1.2

0.26

26.4 ± 1.8

10.62 ± 0.55

10.8 ± 1.3

0.24

25.4 ± 2.1

9.75 ± 0.65

11.1 ± 1.6

0.25

26.0 ± 2.5

9.92 ± 0.49

10.7 ± 0.9

0.24

24.1 ± 2.5

9.72 ± 0.98

K-8

10.8 ± 0.7

0.24

27.6 ± 2.5

11.87 ± 0.34

10.5 ± 0.5

0.24

26.4 ± 2.8

10.92 ± 0.64

10.4 ± 0.9

0.24

27.0 ± 1.9

11.11 ± 0.62

10.5 ± 1.2

0.24

25.2 ± 1.8

10.87 ± 0.74

  1. The culture media was CgXIIIP-medium with 40 g/L of glucose, fructose, beet molasses or 20 g/L of sucrose as sole carbon source
  2. DCW: Dry cell weight (g/L); μmax.: The maximal specific growth rate (/h); PLys: The production of l-lysine (g/L); qs: The substrate consumption rate (qs; mmol C/(g DCW)/h)
  3. aThe data was based on the sucrose consumption rate