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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Redesign and reconstruction of a steviol-biosynthetic pathway for enhanced production of steviol in Escherichia coli

Fig. 3

Functional complementation of native KOs and N-terminally engineered KOs of A. thaliana and S. rebaudiana. SDS-PAGE analysis of the expression of these KOs is presented too. a The production of ent-kaurenoic acid in the MGIUK strains expressing SrKO, trSrKO, AtKO, trAtKO, and UtrAtKO. The results represent means from five independent experiments. b SDS-PAGE analysis [in a 10% (w/v) gel] of a crude protein extract of E. coli BL21 (DE3) harboring pET21α (+), pET21α (+)_AtKO, and pET21α (+)_trAtKO. M: protein molecular weight markers, lane 1: pET21α (+), lane 2: pET21α (+)_AtKO, and lane 3: pET21α (+)_trAtKO

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Microbial Cell Factories

ISSN: 1475-2859

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