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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

Fig. 5

Transient expression of T. wilfordii CYP712K1, CYP712K2 and CYP712K3 in tobacco leaves for the determination of the exact masses of the products formed. Metabolites formed in in response to the co-expression of the indicated enzymes were detected by LC–ESI–MS analysis (showing EIC: m/z 443.35; 457.35 ± 0.05 [M+H]+) of leaf disc methanol extracts. Co-infiltration with p19 and CfDXS was used to inhibit silencing of foreign gene expression and to elevate the isoprenoid diphosphate substrate pool, respectively

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Microbial Cell Factories

ISSN: 1475-2859

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