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Table 1 Carbon balances and yield coefficients for biomass (X), CO2, acetate (Ac), formate (For) and malate (Mal) with the substrate glucose (S) of batch cultivations in stirred-tank bioreactors of the two triple reporter strains E. coli G7BL21(DE3) and E. coli G5T7E2 in comparison to wildtypes E. coli BL21(DE3) and E. coli T7E2 with minimal medium

From: Development and characterization of Escherichia coli triple reporter strains for investigation of population heterogeneity in bioprocesses

Strain

BL21(DE3)

G7BL21(DE3)

T7E2

G5T7E2

Yield, Cmole Cmole−1

 YXS

0.64 ± 0.03

0.68 ± 0.03

0.68 ± 0.04

0.69 ± 0.02

 YCO2S

0.19 ± 0.02

0.16 ± 0.01

0.18 ± 0.02

0.19 ± 0.02

 YAcS

0.08 ± 0.01

0.08 ± 0.02

0.03 ± 0.01

0.03 ± 0.03

 YForS

0.06 ± 0.02

0.04 ± 0.02

0.07 ± 0.03

0.07 ± 0.02

 YMalS

0.04 ± 0.02

0.02 ± 0.02

0.03 ± 0.01

0.02 ± 0.01

Carbon balance

1.01 ± 0.03

0.98 ± 0.03

0.99 ± 0.03

0.99 ± 0.02

  1. YXS biomass yield with glucose, YCO2S yield of CO2 with glucose, YAcS yield of acetate with glucose
  2. All values are given as mean with error bars originating from biological triplicates