Fig. 9
Mean fluorescence (left) and coefficient of variance (right) of the triple reporter strains E. coli G7 BL21(DE3) (grey squares) and E. coli G5 T7E2 (black circles) following perturbation with 2 g L−1 concentrated glucose and simultaneous interruption of air supply for 30 min in aerobic glucose-limited chemostat cultures with minimal medium in stirred-tank bioreactors at 0.2 h−1 (T = 37 °C, pH = 6.8, initial glucose concentration 20 g L−1). Values are shown for single cell growth related to rrnB-EmGFP expression (a), general stress response of single cells related to rpoS-mStrawberry expression (b) and oxygen limitation of single cells related to nar-TagRFP657 expression (c) as average of triplicate measurements. Grey areas illustrate the period without air supply whereas dashed lines indicate when the pulsed glucose was depleted (around 50 min after perturbation with E. coli G7BL21(DE3) (grey line) and around 45 min after perturbation with E. coli G5T7E2 (black line)). Error bars indicate triplicate datasets