Table 4 Oligonucleotide primers used for site-directed mutagenesis. Mutations introduced are shown in bold. NdeI and XhoI restriction sites are underlined
Primers | Oligonucleotides sequences |
|---|---|
OaCLH-F | 5’-CATATGAGCGTTATCTCTCGTCACTCTAGC-3’ |
OaCLH-R | 5’-CTCGAGTTACTGAACGGTCTGGCCCTGAAA-3’ |
S159A | 5’-TCTGCTGGGTCACGCTTGGGGTGGCGCGG-3’ |
D224N | 5’-GGCCTGATCGCGGGTAATCGTGATGGCGTT-3’ |
D226N | 5’-TCGCGGGTGATCGTAATGGCGTTATCCAGG-3’ |
H258A | 5’-TTCGTGGCGCTAACGCTTACGGTATCACCA-3’ |