Fig. 2
Effect of expression conditions on the catalytic efficiency of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh, E. coli pET-21b-laad/pET-28a-dapdh-fdh, and E. coli pET-28a-dapdh-fdh-laad. a Effect of the IPTG concentration on the catalytic activity of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh. The induction temperature was 17 °C. b Effect of the IPTG concentration on the catalytic activity of E. coli pET-21b-laad/pET-28a-dapdh-fdh. The induction temperature was 17 °C. c Effect of the IPTG concentration on the catalytic activity of E. coli pET-28a-dapdh-fdh-laad. The induction temperature was 17 °C. d Effect of the induction temperature on the catalytic activity of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh. The IPTG concentration was 0.75 mM. e Effect of the induction temperature on the catalytic activity of E. coli pET-21b-laad/pET-28a-dapdh-fdh. The IPTG concentration was 1.5 mM. f Effect of the induction temperature on the catalytic activity of E. coli pET-28a-dapdh-fdh-laad. The IPTG concentration was 0.75 mM. The reaction mixture contained 50 mg/mL whole-cell biocatalyst, NADP+ (5 mM), NH4Cl (150 mM), sodium formate (100 mM) and l-Phe (50 mM). Reactions were all performed in Tris-HCl buffer (50 mM, pH 9.0) at 30 °C and 220 rpm for 6 h. The values were averaged from triplicate measurements