Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Highly selective synthesis of d-amino acids via stereoinversion of corresponding counterpart by an in vivo cascade cell factory

Fig. 1

Construction of a multi-enzymatic cascade system by the regulation of enzyme expression. a Gene expression optimization with plasmids for the biotransformation of l-Phe into d-Phe. Reactions were carried out in Tris-HCl buffer (50 mM, pH 9.0) at 30 °C. The reaction mixture contained 50 mg/mL whole-cell biocatalyst, NADP+ (3 mM), NH4Cl (90 mM), sodium formate (60 mM), and l-Phe (30 mM). All reactions were carried out in Tris-HCl buffer (50 mM, pH 9.0) at 30 °C and 220 rpm. The values were averaged from triplicate measurements. b SDS-PAGE of recombinant E. coli pET-28a-laad-dapdh-fdh (1), E. coli pET28a-dapdh-fdh-laad (2), E. coli pET21b-laad/pET-28a-dapdh-fdh (3), and E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh (4). T: total cell lysate; S: soluble fraction. The samples were generated from 50 mg/mL E. coli

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us