Fig. 4
2,4-PDCA production (analysed by HPLC) for R. jostii pcaHG:ligAB(Ptpc5) and R. jostii pcaHG:ligAB(Ptpc5)/pTipQC2-dyp2 grown on 4 mL scale on M9 media containing different carbon sources and media additives. a Growth on M9 minimal media containing different lignocellulose or lignin feedstocks, with or without expression of Amycolatopsis dyp2 gene, for 168 h at 30 °C. b Growth on M9 media containing 0.2% Green Value lignin with or without expression of Amycolatopsis dyp2 gene, with addition of 0.15 g/L FeSO4, MnSO4, CuCl2, or all three salts, for 168 h at 30 °C. Error bars are standard deviations from triplicate biological replicates