Fig. 5
From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8
Purification and analysis of aFGF. a The overall purification steps. b SDS-PAGE analysis of samples from each purification step. c C18 RP-HPLC trace. d LC–MS (Q-TOF) analysis. M: marker; Lys: supernatant after sonication; 1: HisTrap purification; 2: after TEV protease treatment (the band at 28 kDa is TEVp); 3: HisTrap purification; 4: HiTrap CM purification (final product). The data are representative of three replicated experiments