Fig. 7

The synthesis of l-tle catalyzed by high content of fusion enzyme whole cells. a 2 mL reaction system including 40 g/L whole cells, different concentrations of substrate and 0.4 mM NAD⁺ under 30 °C, pH 9.0, 200 rpm. 500 mM (green), 400 mM (wathet), 300 mM (blue), 200 mM (purple), 100 mM (orange). b 200 mL reaction system including 40 g/L whole cells, 500 mM substrate and 0.4 mM NAD⁺ under 30 °C, pH 9.0, 200 rpm. Yield (squares), productivity (circles), e.e. (triangles). Data represent the mean ± standard deviation of duplicate samples