Fig. 6

The synthesis of l-tle catalyzed by fusion enzyme whole cells. a 2 mL reaction system including 8 g/L whole cells, 500 mM substrate and 0.4 mM NAD⁺ under 30/40/50 °C, pH 9.0, 200 rpm. b 2 mL reaction system including 8 g/L whole cells, 500 mM substrate and 0.4 mM NAD⁺ under 30 °C, pH 8.0/9.0/10.0, 200 rpm. c 2 mL reaction system including 8 g/L whole cells, 100 mM substrate and different kinds of cofactor under 30 °C, pH 9.0, 200 rpm. d 2 mL reaction system including 8 g/L whole cells, 500 mM substrate and different concentrations of NAD⁺ under 30 °C, pH 9.0, 200 rpm. e 2 mL reaction system including 8 g/L whole cells, different concentrations of substrate and 0.4 mM NAD⁺ under 30 °C, pH 9.0, 200 rpm. Productivity (triangles), concentration (squares), yield (circles). (f) 2 mL reaction system including different concentrations of whole cells, 500 mM substrate and 0.4 mM NAD⁺ under 30 °C, pH 9.0, 200 rpm. Productivity indicates the concentration of l-tle synthesized in 1 h. Data represent the mean ± standard deviation of duplicate samples