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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Improved production of germacrene A, a direct precursor of ß-elemene, in engineered Saccharomyces cerevisiae by expressing a cyanobacterial germacrene A synthase

Fig. 1

Schematic overview of germacrene A production in S. cerevisiae. To improve germacrene A production, GAS (marked in red) screening and site-directed mutagenesis (represented with an asterisk) were performed, the tHMGR1 gene (marked in purple) was overexpressed, the LPP1 and DPP1 genes (marked in green) were deleted, and the ERG9 gene (marked in blue) was downregulated by replacing its native promoter with the promoter of HXT1. Acetyl-CoA: acetyl coenzyme A, HMG-CoA: 3-hydroxy-3-methylglutaryl-CoA, tHMGR1: truncated HMG-CoA reductase gene, IPP: isopentenyl pyrophosphate, DMAPP: dimethylallyl pyrophosphate, ERG20: farnesyl diphosphate synthase gene, FPP: farnesyl diphosphate, FOH: farnesol, ERG9: squalene synthase gene, GAS: germacrene A synthase. Solid arrows represent one-step transformation while dashed arrows represent transformation comprising multiple intermediate steps

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Microbial Cell Factories

ISSN: 1475-2859

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