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Table 1 Fold change of AppA phytase production relative to MDP PAOX1, PHpFMD or BDP PHpFMD-HpMOX 

From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Strain

Fold change relative to AppA (PAOX1)

Fold change relative to AppA (PHpFMD)

Fold change relative to AppA-PDI (PHpFMD-HpMOX)

AppA (PHpFMD)

1.53 ± 0.01*

–

–

AppA-HAC1 (PHpFMD-HpMOX)

2.08 ± 0.07*

1.36 ± 0.05**

–

AppA-HAC1 (PAOX1-CAT1)

1.31 ± 0.09*

0.86 ± 0.06**

–

AppA-HAC1 (PDAS1/2)

0.54 ± 0.03*

0.35 ± 0.02**

–

AppA-PDI (PHpFMD-HpMOX)

2.14 ± 0.03*

1.40 ± 0.02**

–

AppA-PDI (PAOX1-CAT1)

1.71 ± 0.07*

1.12 ± 0.05**

0.80 ± 0.03***

AppA-PDI (PDAS1/2)

1.53 ± 0.50*

1.00 ± 0.13

0.71 ± 0.09***

AppA-MPDI (PHpFMD-HpMOX)

0.68 ± 0.16*

0.44 ± 0.10**

–

AppA-EUG1 (PHpFMD-HpMOX)

1.37 ± 0.02*

0.90 ± 0.01**

–

AppA-SEC1 (PHpFMD-HpMOX)

1.54 ± 0.04*

1.01 ± 0.02

–

AppA-SLY1 (PHpFMD-HpMOX)

1.63 ± 0.01*

1.06 ± 0.01

–

AppA-ERV2 (PHpFMD-HpMOX)

2.38 ± 0.03*

1.56 ± 0.02**

1.11 ± 0.01***

AppA-ERO1 (PHpFMD-HpMOX)

0.79 ± 0.11*

0.52 ± 0.07**

0.38 ± 0.05***

AppA-PDI-ERO1 (PGAP)

2.59 ± 0.12*

1.70 ± 0.08**

1.21 ± 0.06***

AppA-PDI-ERO1 (PCAT1)

2.33 ± 0.24*

1.52 ± 0.16**

1.09 ± 0.11

AppA-PDI-ERV2 (PGAP)

2.90 ± 0.08*

1.90 ± 0.05**

1.35 ± 0.04***

AppA-PDI-ERV2 (PCAT1)

2.42 ± 0.12*

1.59 ± 0.08**

1.13 ± 0.05

AppA-PDI-GPX1 (PGAP)

1.71 ± 0.01*

1.12 ± 0.01

0.80 ± 0.01***

AppA-PDI-GPX1 (PCAT1)

2.8 ± 0.31*

1.83 ± 0.20**

1.30 ± 0.14***

AppA-PDI-HAC1 (PCAT1)

2.26 ± 0.35*

1.48 ± 0.16**

1.06 ± 0.16

AppA-HAC1-PDI (PGAP)

1.72 ± 0.23*

1.13 ± 0.15

0.80 ± 0.11***

AppA-PDI-KAR2 (PGAP)

2.01 ± 0.01*

1.32 ± 0.01**

0.94 ± 0.02

  1. aAll strains have a single copy of the integrated cassette (CNV = 1) as determined by ddPCR
  2. *Significantly different from AppA (PAOX1) (p value Ë‚ 0.05)
  3. **Significantly different from AppA (PFMD) (p value Ë‚ 0.05)
  4. ***Significantly different from AppA-PDI (PHpFMD-HpMOX) (p value Ë‚ 0.05)