Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Fig. 2

HAC1 or PDI co-expression from BDPs further increases phytase yields. a AppA co-expressed with HAC1 under PAOX1-CAT1, PDAS1/2 and PHpFMD-HpMOX. b AppA co-expressed with PDI under the BDPs PAOX1-CAT1, PDAS1/2 and PHpFMD-HpMOX. c AppA co-expressed with MPDI and EUG1 under the BDP PHpFMD-HpMOX. The AppA-HAC1 (PHpFMD-HpMOX) and AppA-PDI (PHpFMD-HpMOX) strains were included for comparison. Phytase expression was determined by the pNPP assay after methanol induction. Data are represented as mean values ± standard deviation (n = 3)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us