Fig. 1From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastorisAppA expression from PHpFMD surpasses P. pastoris endogenous MDPs (PAOX1, PDAS1, PCAT1 and PGAP). Phytase expression was determined by the p-NPP assay. All strains except AppA (PGAP) were induced with methanol at 65 h and consecutive additions until 132 h. Data are represented as mean values ± standard deviation (n = 3)Back to article page