Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei

Table 3 Steady-state kinetic constants of PcCDH, PcCDH_Tr and PcCDH_Pp

Electron donors	This study: PcCDH_Tr^a			Published: PcCDH				Published: PcCDH_Pp
	K_M^b	k_cat^b	k_cat/K_M^b	K_M	k_cat	k_cat/K_M	Refs.	K_M	k_cat	k_cat/K_M	Refs.
	[mM]	[s⁻¹]	[mM⁻¹ s⁻¹]	[mM]	[s⁻¹]	[mM⁻¹ s⁻¹]	Refs.	[mM]	[s⁻¹]	[mM⁻¹ s⁻¹]	Refs.
Cellobiose	0.08 ± 0.008	27.8 ± 0.9	340 ± 35	0.04	25.7	643	[32]	0.06^e	40^e	689^e	[16]
Cellobiose	0.08 ± 0.008	27.8 ± 0.9	340 ± 35	0.11	15.7	143	[14]	0.06	3.6^f	60.3^f	[24]
Lactose	1.42 ± 0.08	32.3 ± 1.9	22.7 ± 1.8	1.1	13.4	12	[14]	1.16	4.7^f	4.1^f	[24]
Glucose	2109 ± 171	7.8 ± 0.5	0.004 ± 0.0004	1600	2.64	0.0017	[14]

Electron acceptors	K_M	k_cat	k_cat/K_M	K_M	k_cat	k_cat/K_M	Refs.	K_M	k_cat	k_cat/K_M	Refs.
Electron acceptors	[mM]	[s⁻¹]	[mM⁻¹ s⁻¹]	[mM]	[s⁻¹]	[mM⁻¹ s⁻¹]	Refs.	[mM]	[s⁻¹]	[mM⁻¹ s⁻¹]	Refs.
Cytochrome c	0.00036 ± 0.00009^c	13.0 ± 0.2^c	36,143 ± 8618^c	0.001	20.5	17,083	[6]	0.001	37.3	25,903	[16]
Cytochrome c	0.00036 ± 0.00009^c	13.0 ± 0.2^c	36,143 ± 8618^c	0.0007	14.2	20,286	[32]	0.001	37.3	25,903	[16]
DCIP	0.00431 ± 0.00011^d	24.4 ± 0.2^d	5655 ± 159^d	0.004	33	9167	[6]
DCIP	0.00431 ± 0.00011^d	24.4 ± 0.2^d	5655 ± 159^d	0.006	27	4219	[32]

ISSN: 1475-2859