Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii

Table 3 Comparison of translocon demands by ontological function

	Genes (n)	Nascent^a (%)	Ribosomes^b (%)
Cotranslationally translocated^c
Function unknown	261	8.00	11.0
Cell wall/membrane/envelope Biogenesis	41	7.00	12.0
Post-translational modification, protein turnover and chaperones	89	7.00	12.0
Carbohydrate transport and metabolism	114	7.00	9.0
Intracellular trafficking, secretion and vesicular transport	95	6.00	7.0
Inorganic Ion transport and metabolism	82	5.00	10.0
Lipid transport and metabolism	72	4.00	5.0
Posttranslationally translocated^d
Function unknown	30	36	11.0
Cell wall/membrane/envelope Biogenesis	10	15	10.0
Post-translational modification, protein turnover and chaperones	5	0	0.0

^aCalculated as percent of total cTPM for all proteins predicted to be ER destined
^bCalculated as percent of total cRPM for all proteins predicted to be ER destined
^cProteins with greater than twofold membrane enrichment and not predicted to be mitochondrial
^dProteins with less than twofold membrane enrichment and not predicted to be mitochondrial and contained a predicted signal sequence

ISSN: 1475-2859