Table 1 List of primers used for cloning
S.No | Name | Sequence (5′ → 3′) |
|---|---|---|
Primers used for cloning of Rubella-sfGFP | ||
 1 | Rubella Fw + overhang | GAGATATACATATGCATATGCTGTCAGTCGCAGGCGT |
 2 | Rubella Rv + overhang | CACAGCCGTGGTATGCGTACCCCGCATAGATCTCGATCGAGAACCACCACCACC |
 3 | sfGFPFw + overhang | ACGGCTGTGGGTGGTGGTGGTTCTCGATCGAGATCTATGCGGGGTTCTCATCATCA |
 4 | sfGFPRv + overhang | TTACTTGTACAGCTCGTCCATGTGCCTGCAGGTCGACTCTAG |
 5 | Vector Fw + overhang | TGGACGAGCTGTACAAGTAACTAGAGTCGACCTGCAGGCATGCA |
 6 | Vector Rv + overhang | ACGCCTGCGACTGACAGCATATGCATATGTATATCTC |
Primers used for cloning of glycerol metabolism genes | ||
 1 | FW glpDvec overhang | TGGAGATGACGATGACAAGGTGGTCGACAAGCTTATGGAAACCAAAGATCTGATTGT GATAGG |
 2 | RV glpDara overhang | CGCTAATCTTATGGATAAAAATGCTATGCTCGATTACGACGCCAGCGATAACCTCT |
 3 | FW AraglpD overhang | TATACGCAGCAGAGGTTATCGCTGGCGTCGTAATCGAGCATAGCATTTTTATCCATA AGATTAG |
 4 | RV AraglpK overhang | TGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGGGTACCTTTCTCCTCTTTAATG AATTCT |
 5 | FW glpKara overhang | TCACACAGAATTCATTAAAGAGGAGAAAGGTACCCATGACTGAAAAAAAATATATCG TTGCGCTC |
 6 | RV glpKvec overhang | CCGCATCGATCGGGCCCTGAGGCCTGCAGGGATCCTTATTCGTCGTGTTCTTCCCA CGC |
 7 | FW vecglpK overhang | AACGCGCGATGGCGTGGGAAGAACACGACGAATAAGGATCCCTGCAGGCCTCAGG GCC |
 8 | RV vecglpD overhang | CGCCCCCTATCACAATCAGATCTTTGGTTTCCATAAGCTTGTCGACCACCTTGTCAT CGTCATCTC |
 9 | glpKFw | CCAAGCTTATGACTGAAAAAAAATATATCG |
 10 | glpKRv | GCTCTAGATTATTCGTCGTGTTCTTC |
 11 | glpDFw | GCAAGCTTATGGAAACCAAAGATCTGATTG |
 12 | glpDRv | AATCTAGATTACGACGCCAGCGATAACC |