Fig. 8
Effect of glycerol pulsing on glycerol uptake and product yield. Experiments were conducted in TB medium containing with 0.2% v/v glycerol and 10 mM MgSO4. The DKO strain transformed only with the pMALS1Asp plasmid (for L-asparaginase expression) was used as control (red), and DKO strain co-transformed with pMALS1Asp and pPROLAR.A122glpDK plasmids but induced only for L-asparaginase expression was used as test (green). a Growth profile of control and test cultures (O.D. measurements at 600 nm). b Glycerol utilization profile for control and test cultures upon continuous glycerol pulsing (0.3% v/v) at regular intervals of 3 h, starting from 6 h post induction till 12 h post induction. A final glycerol pulse (0.2% v/v) was added at 21 h post induction and (c) Product concentration (mg/L) at 12 h and 24 h for control and test cultures. The data represents mean ± SD of three independent biological replicates