Fig. 7

Growth, glycerol consumption and product concentration of DKO strain co-transformed with substrate utilization genes ‘glpDK’. The expression of recombinant L-asparaginase was tested in TB medium supplemented with 0.2% v/v glycerol and 10 mM MgSO₄. The DKO strain transformed only with the pMALS1Asp plasmid (for L-asparaginase expression) was used as control (red). The test DKO cultures were co-transformed with both pMALS1Asp and pPROLAR.A122glpDK plasmids (for expression of substrate utilization genes glpK and glpD) but induced only for L-asparaginase expression (green), or induced for both plasmids (sky-blue). a Growth profiles of control and test (O.D. measurement at 600 nm); (b) Glycerol utilization profile; and (c) Product concentration (mg/L) at 10 h and 24 h, for control and test cultures. Data represents mean ± SD of three independent biological replicates