Table 3 Primers used in this study
Primers | Sequence (5′-3′) |
|---|---|
For the construction of deletion vector (pNZ5319-up-down) | |
 Up-f | CCGCTCGAGTTAATCGGTGGTGTTACTACTGG |
 Up-r | CCCATTTAAATTTTAACCGGGGTTTTTGC |
 Down-f | CCCGAGCTCCTGAAGCGGGAGATACAGAAAC |
 Down-r | GAAGATCTCGCTTCAATCTCTCCCAAAGT |
For the identification of mutant strain (L. lactis N8-1) | |
 Inner-f | AAATATGGAATTGAAGCATTTAA |
 Inner-r | TTATTCTTTCGGTTTAGATGACT |
 Exter-f | TGAATAAGAATTTGAACCCTTTA |
 Exter-r | AGAAATTCTCTGATAAATTTTCTG |
 Cat-f | TCAAATACAGCTTTTAGAACTGG |
 Cat-r | TACAGTCGGCATTATCTCATATTA |
 Ery-f | CTTGCTCATAAGTAACGGTAC |
 Ery-r | CGATACCGTTTACGAAATTGG |
For the RT-qPCR | |
 Q-tufA-f | GACCTCTTGAGCGAATACGACT |
 Q-tufA-r | TTCTTCAACTTTAGCAACCCATT |
 Q-rpsN-f | CCTGCAAAATTCTCAACACAAGC |
 Q-rpsN-r | GCGAAGACAGATACGGCAAAGT |
 Q-rplR-f | TGGTACTAAAACTGAACAAGCCG |
 Q-rplR-r | TGCAACACGTCCGTGATAGAGGT |
 Q-csc2B-f | ACAGGAGGAGCACTTTCAATCG |
 Q-csc2B-r | GGTCCATCCATCCCAGGTT |
 Q-csc2C-f | GCCAGTTTACAGGCACAGGTC |
 Q-csc2C-r | GGATTAGCGTCATTCGTAGCATT |
 Q-pi339-f | TAGTCGCAGCAGGAATCTTTG |
 Q-pi339-r | TATCTTGATTGATTTGGTCTTTC |
 Q-pp423-f | CGCAATAAGAAGAATGCCAAGC |
 Q-pp423-r | GATGCCCCAGCGATCAATA |
 Q-butA-f | ATTATCAACGCAACCTCACAAGC |
 Q-butA-r | TCCCCATTCATCATCTTTACCAG |
 Q-butB-f | CCGTCAGCAGAACATCCTAATC |
 Q-butB-r | TCAGCCAATCCTCCACCAT |
 Q-arcA-f | AAGCGGGTTACCGTCCAG |
 Q-arcA-r | TTGGTAATGGGTTGAGGTAGAAA |
 Q-arcB-f | GCTCCAGATTCACTTCATCCTAC |
 Q-arcB-r | TCTTCCCAGTTAGATTCTCCCAT |
 Q-galM-f | AATTGTTCGTGGTGATATCGTTG |
 Q-galM-r | ATCACAATACTTGGTTGGTCGGT |
 Q-galK-f | TCCTATTGGACTGTAACACTCTA |
 Q-galK-r | TTCATCCCCAATCAAATCAGTAT |
 Q-lacZ-f | CGGTCCGCTGCTCTCATTATCCT |
 Q-lacZ-r | AGTCATTCCGTGCGTTTCG |