Table 1 Strains and plasmids used in this study

Strains

 S. cerevisiae BY4742

MATα; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0

Novagen

 4742–301

BY4742 with pCf301

This study

 4742-UGT73B6^FSy

BY4742 with pCf301-UGT73B6^FSy

This study

 4742-AsUGT^syn

BY4742 with pCf301-AsUGT^syn

This study

 4742-pCf

BY4742 with pCf301& pCf302

This study

 4742-pGS

BY4742 with pCf302-CP & pCf301-AUA

This study

 S. cerevisiae aro7Δ

The deletion of ARO7 in S. cerevisiae BY4742

This study

 Δaro7-pCf

S. cerevisiae aro7Δ with pCf301 & pCf302

This study

 Δ7-pGS

S. cerevisiae aro7Δ with pCf302-CP & pCf301-AUA

This study

 rGS3

P_TDH3-PPTcg-1^syn-T_TEF-P_ScTEF1-CAR^syn-T_CYC1, URA3-T_ADH1-ARO4^K229L-P_PGK1-P_TDH3-ubiC^syn-T_TEF-P_ScTEF1-AsUGT^syn-T_CYC1 cassettes were integrated into rDNA locus of S. cerevisiae aro7Δ

This study

 Δ7-HBA

P_TDH3-ARO2-T_TEF-P_ScTEF1-ARO1-T_CYC1, LEU2-T_ADH1-ARO4^K229L-P_PGK1-P_TDH3-ubiC^syn-T_TEF, P_TDH3-ppsA-T_TEF-P_ScTEF1-tktA-T_CYC1 cassettes were integrated into δ sites of S. cerevisiae aro7Δ

This study

 rGS-HBA

P_TDH3-ARO2-T_TEF-P_ScTEF1-ARO1-T_CYC1, LEU2-T_ADH1-ARO4^K229L-P_PGK1-P_TDH3-ubiC^syn-T_TEF, P_TDH3-ppsA-T_TEF-P_ScTEF1-tktA -T_CYC1 cassettes were integrated into δ sites of rGS3

This study

Plasmids

 pCf301

URA3; 2 μm ori with P_PGK1, P_TDH3, P_TEF1; Amp

In our lab ³⁰

 pCf302

LEU2; 2 μm ori with P_PGK1, P_TDH3, P_TEF1; Amp

In our lab ³⁰

 pCf301-UGT73B6^FSy

pCf301 carrying P_ScTEF1-UGT73B6^FSy-T_CYC1

This study

pCf301- AsUGT^syn

pCf301 carrying P_ScTEF1-AsUGT^syn-T_CYC1

This study

 pCf302-CP

pCf302 carrying P_ScTEF1-CAR^syn -T_CYC1, P_TDH3-PPTcg-1^syn -T_TEF

This study

 pCf301-AUA

pCf301 carrying P_PGK1-ARO4^K229L-T_ADH1, P_TDH3-ubiC^syn -T_TEF, P_ScTEF1-AsUGT^syn-T_CYC1

This study

 pCf302-AU

pCf302 carrying P_PGK1-ARO4^K229L-T_ADH1, P_TDH3-ubiC^syn -T_TEF

This study

 pCf301-pt

pCf301 carrying P_TDH3-pps -T_TEF-P_ScTEF1-tktA-T_CYC1

This study

 pCf301-ARO1/2

pCf301 carrying P_TDH3-ARO-T_TEF-P_ScTEF1-ARO1-T_CYC1

This study