Fig. 6

Titers of gastrodin, 4-hydroxybenzyl alcohol and 4-HBA, and the cell density of engineered strains. The strain 4742-pGS, S. cerevisiae BY4742 with plasmid pCf302-CP and pCf301-AUA (carrying CAR^syn, PPTcg-1^syn, ARO4^K229L, ubiC^syn and AsUGT^syn); the strain Δ7-pGS, S. cerevisiae aro7Δ with plasmids pCf302-CP and pCf301-AUA (carrying CAR^syn, PPTcg-1^syn, ARO4^K229L, ubiC^syn and AsUGT^syn); the strain rGS3, with genes of CAR^syn, PPTcg-1^syn, ARO4^K229L, ubiC^syn and AsUGT^syn integrated into rDNA locus of S. cerevisiae aro7Δ; the strain Δ7-HBA, with genes of ARO2, ARO1, ARO4^K229L, ubiC^syn, ppsA and ktA integrated into δ sites of S. cerevisiae aro7Δ; the strain rGS-HBA with genes of ARO2, ARO1, ARO4^K229L, ubiC^syn, ppsA and ktA integrated into δ sites of strain rGS3. Three replicates were performed, and the error bars represented standard deviation. The significance differences of cell density between the control Δ7-pGS and other engineered strain were analyzed by Student’s t-test. a, p-value < 0.01; b, p-value < 0.05; c, no statistical significance differences