Isolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14

Table 1 Antifungal activity of recombinant protein rF2 treated under different temperatures

Temperature/°C	Diameter of inhibition zone/mm	Rate of activity loss (%)
CK	13.37 ± 0.12a	–
40	13.31 ± 0.11a	0.4
60	13.29 ± 0.18a	0.6
80	13.11 ± 0.16a	1.9
100	12.23 ± 0.15b	8.5
121	10.46 ± 0.10c	21.8

CK is the negative control comprising a 30 µl aliquot of 10 mmol/l Tris–HCl buffer (pH 7.5). The means within a column followed by different letters are significantly different from each other at P = 0.05

ISSN: 1475-2859