Fig. 2

Purification of antifungal protein F2. Elution profile of fraction F obtained using reversed phase chromatography on a SOURCE™ 5RPC 4.6/150 column (a). The antifungal activities of the separated proteins against Rhizoctonia cerealis (b) and the purity and molecular mass of the purified antifungal protein F2 on SDS-PAGE (c). F1–F4 represent separated components from fraction F obtained using DEAE Sepharose Fast Flow ion-exchange chromatography [15 µg of each separated fraction dissolved in 30 µl of 10 mmol/l Tris–HCl buffer (pH 7.5)]; CK represents the negative control comprising 30 µl of 10 mmol/l Tris–HCl buffer (pH 7.5); lane 1: molecular weight marker; lane 2: the purified antifungal protein F2 from column chromatography