Different transcriptional responses of haploid and diploid S. cerevisiae strains to changes in cofactor preference of XR

Table 1 Fermentation characteristics of strains using YPX50 with an initial inoculum of 4 g/L DCW

	Q ^a (g/L/h)		Y ^b (g/g consumed xylose)			Enzymatic activity (U/mg) ^d			[NADPH]/[NADH] ^e
	Xylose	Ethanol	Xylitol	Ethanol	Biomass ^c	NADPH-XR	NADH-XR	XDH	[NADPH]/[NADH] ^e
HX57D	2.58 ± 0.01	0.83 ± 0.01	0.09 ± 0.01	0.32 ± 0.00	0.04 ± 0.00	0.65 ± 0.03	0.86 ± 0.01	11.24 ± 0.72	0.76
HX62W	1.93 ± 0.01	0.55 ± 0.01	0.21 ± 0.01	0.28 ± 0.01	0.07 ± 0.00	0.52 ± 0.03	0.36 ± 0.02	6.61 ± 0.09	1.45
A	2.54 ± 0.04	0.83 ± 0.00	0.15 ± 0.00	0.33 ± 0.00	0.11 ± 0.00	0.10 ± 0.00	0.31 ± 0.01	2.30 ± 0.00	0.31
B	2.58 ± 0.07	0.50 ± 0.01	0.39 ± 0.01	0.19 ± 0.00	0.07 ± 0.00	0.44 ± 0.01	0.26 ± 0.00	3.83 ± 0.14	1.67

Data for enzymatic activity were measured at 8 h of fermentation. Other data were calculated by 16 h fermentation. Data are means ± SD from three independent experiments
^aXylose consumption rate or ethanol production rate (g/L/h)
^bYield of xylitol and ethanol (g/g consumed xylose)
^cYield of biomass (g DCW/g consumed xylose)
^dSpecific activity (U/mg protein)
^eSpecific activity with NADPH/specific activity with NADH

ISSN: 1475-2859