Table 2 Bacterial strains and plasmids used in this study
From: Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis
Strain or plasmid | Genotype or description | Reference | |
|---|---|---|---|
Strains | |||
 Escherichia coli | |||
  JM109 | mcrA recA1 supE44 endA1 hsdR17 (rK–mK+) gyrA96 relA1 thi ∆(lac-proAB) F' [traD36 proAB+ lacIq lacZ ∆M15] | [48] | |
 Bacillus subtilis | |||
  JABs24 | B. subtilis 168 ΔmanPA; trp + ; sfp + ; | [49] | |
  3NA | spo0A3; | [50] | |
  JABs32 | spo0A3; ΔmanPA::erm; sfp + ; | J. Altenbuchner (unpublished) | |
  BMV9 | spo0A3; ΔmanPA; sfp + ; | This study | |
  BMV10 | spo0A3; ΔmanPA; sfp + ; ΔamyE:: degQ (from B. subtilis DSM10T) | This study | |
  BMV11 | spo0A3; ΔmanPA; sfp + ; Ppps-ppsA-E:: Pveg–ppsA-E | This study | |
  BMV12 | spo0A3; ΔmanPA; sfp + ; ΔsrfAA-AD:: comS-erm | This study | |
  BMV13 | spo0A3 ΔmanPA; sfp + ; ΔsrfAA-AD:: comS -erm; Ppps-ppsA-E::Pveg–ppsABCDE | This study | |
  BMV14 | spo0A3; ΔmanPA; sfp + ; Ppps-ppsABCDE::Pveg–ppsABCDE; ΔamyE::degQ (from B. subtilis DSM10T) | This study | |
Plasmids | |||
 pJOE6743.1 | oripUC18, bla, spc, manP, ter-lacI-lacZα-ter | [51] | |
 pJOE7644.2 | oripUC18, bla,PmanP-manP, spc, manR-ctaO | [46] | |
 pJOE4786.1 | oripUC18, bla, ter-′lacI-lacZα-ter | [52] | |
 pKAM312 | oripBR322, rop, ermC, bla, amyE′-[ter-PglcR-lacZ-spcR]- ′amyE | [46] | |
 pMAV3 | pJOE4786.1 containig Pveg::Ppps exchange fragment (integrated by Sma I) | This study | |
 pMAV4 | pJOE6743.1 containig Pveg::Ppps exchange fragment (integrated by Hind III) | This study | |
 pMAV5 | pKAM312 containing degQ (B. subtilis DSM10T) (integrated by Hind III) | This study | |