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Table 2 Bacterial strains and plasmids used in this study

From: Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis

Strain or plasmid Genotype or description Reference
Strains
 Escherichia coli
  JM109 mcrA recA1 supE44 endA1 hsdR17 (rKmK+) gyrA96 relA1 thi ∆(lac-proAB) F' [traD36 proAB+ lacIq lacZ ∆M15] [48]
 Bacillus subtilis
  JABs24 B. subtilis 168 ΔmanPA; trp + ; sfp + ; [49]
  3NA spo0A3; [50]
  JABs32 spo0A3; ΔmanPA::erm; sfp + ; J. Altenbuchner (unpublished)
  BMV9 spo0A3; ΔmanPA; sfp + ; This study
  BMV10 spo0A3; ΔmanPA; sfp + ; ΔamyE:: degQ (from B. subtilis DSM10T) This study
  BMV11 spo0A3; ΔmanPA; sfp + ; Ppps-ppsA-E:: PvegppsA-E This study
  BMV12 spo0A3; ΔmanPA; sfp + ; ΔsrfAA-AD:: comS-erm This study
  BMV13 spo0A3 ΔmanPA; sfp + ; ΔsrfAA-AD:: comS -erm; Ppps-ppsA-E::PvegppsABCDE This study
  BMV14 spo0A3; ΔmanPA; sfp + ; Ppps-ppsABCDE::PvegppsABCDE; ΔamyE::degQ (from B. subtilis DSM10T) This study
Plasmids
 pJOE6743.1 oripUC18, bla, spc, manP, ter-lacI-lacZα-ter [51]
 pJOE7644.2 oripUC18, bla,PmanP-manP, spc, manR-ctaO [46]
 pJOE4786.1 oripUC18, bla, ter-′lacI-lacZα-ter [52]
 pKAM312 oripBR322, rop, ermC, bla, amyE′-[ter-PglcR-lacZ-spcR]- ′amyE [46]
 pMAV3 pJOE4786.1 containig Pveg::Ppps exchange fragment (integrated by Sma I) This study
 pMAV4 pJOE6743.1 containig Pveg::Ppps exchange fragment (integrated by Hind III) This study
 pMAV5 pKAM312 containing degQ (B. subtilis DSM10T) (integrated by Hind III) This study