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Table 1 Plipastatin titers achieved of B. subtilis BMV9 (3NA sfp +) and B. subtilis BMV12 (3NA sfp + plipastatin mono-producer)

From: Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis

Supplementation B. subtilis BMV9 B. subtilis BMV12
Plipastatin (mg/L) Plipastatin (mg/L)
Control 19.1 ± 0.1 n. d.*
Glutamic acid 7.8 ± 0.2 n. d
Glutamine 6.6 ± 0.9 n. d
Isoleucine 10.5 ± 2.6 n. d
Alanine 14.3 ± 0.9 n. d
Threonine 11.3 ± 0.8 n. d
Proline 14.4 ± 1.3 n. d
Ornithine 18.4 ± 2.4 6.3 ± 0.5
  1. Plipastatin titers measured after 48 h cultivation in mineral salt medium supplemented with 30 mM of the indicated amino acids compared to control cultivations (without any amino acid supplementation).
  2. *Not detectable