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Table 1 Plipastatin titers achieved of B. subtilis BMV9 (3NA sfp +) and B. subtilis BMV12 (3NA sfp + plipastatin mono-producer)

From: Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis

Supplementation

B. subtilis BMV9

B. subtilis BMV12

Plipastatin (mg/L)

Plipastatin (mg/L)

Control

19.1 ± 0.1

n. d.*

Glutamic acid

7.8 ± 0.2

n. d

Glutamine

6.6 ± 0.9

n. d

Isoleucine

10.5 ± 2.6

n. d

Alanine

14.3 ± 0.9

n. d

Threonine

11.3 ± 0.8

n. d

Proline

14.4 ± 1.3

n. d

Ornithine

18.4 ± 2.4

6.3 ± 0.5

  1. Plipastatin titers measured after 48 h cultivation in mineral salt medium supplemented with 30 mM of the indicated amino acids compared to control cultivations (without any amino acid supplementation).
  2. *Not detectable