Fig. 2

Non-metrical multidimensional scaling (NMDS) analysis of fecal microbiome data. a Bray–Curtis dissimilarity-based NMDS analysis shows no clustering of the gut microbiome based on diet (blue and red symbols vs. green and orange symbols) or genotype (blue and green symbols vs. red and orange symbols) at week 8. b Bray–Curtis dissimilarity-based NMDS analysis shows the clustering of gut microbiome according to diet (blue and red symbols vs. green and orange symbols) rather than genotype (blue and green symbols vs. red and orange symbols) at week 26. Bray–Curtis dissimilarity-based NMDS analysis was performed on OTUs from the fecal samples of 32 mice at each time point. Each dot represents one of 32 gut microbiomes. The OTUs in the analysis were estimated based on 97% 16S rRNA sequence similarity. Blue, red, green, and orange symbols indicate samples collected from AhRKO mice fed HFD, WT mice fed HFD AhRKO mice fed LFD, and WT mice fed LFD, respectively