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Table 2 Expression plasmids used in this study. Unless specified otherwise, all genes, promoters and terminators in the inserts are from K. marxianus

From: Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

Name Contents/comments Source
pUCC001 AaTEF1pr-SpCas9-ScPHO5t ScTDH3pr-HH-gRNAempty-HDV-ScCYC1t hphMX [51]
pMTU-DO-URA Centromeric plasmid with GFP drop-out; ScURA3 KmARS/CEN7; available from Addgene (#160004) This work
pMTU-DO-HIS Centromeric plasmid with GFP drop-out; ScHIS3 KmARS/CEN7; available from Addgene (#160005) This work
pI1-MTU-DO-HIS Integrative plasmid with GFP drop-out targeting LAC4; ScHIS3; available from Addgene (#160142) This work
pI6-MTU-DO-URA Integrative plasmid with GFP drop-out targeting ARO3; ScURA3; available from Addgene (#160030) This work
pI2-MTU-DO-G418 Integrative plasmid with GFP drop-out, kanMX, targeting integration site I2 [27]; available from Addgene (#160018) This work
pI4-MTU-DO-G418 Integrative plasmid with GFP drop-out, kanMX, targeting integration site I4 (Rajkumar et al., 2019); available from Addgene (#160215) This work
pA1f/A4f-U pMTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TEF1pr-ARO7fbr-INU1t This work
pChor/A4f pMTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TSA1pr-ARO1-KMXK_A03020t PGK1pr-ARO2-LAC4t TEF1pr-ARO7fbr-INU1t This work
pAAA/Phe pMTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TSA1pr-ARO1-KMXK_A03020t PGK1pr-ARO2-LAC4t TEF1pr-ARO7fbr-PDC1t ENO1pr-PHA2-INU1t HSP150pr-ARO9-ScPGK1t This work
pI6-AAA/Phe pI6-MTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TSA1pr-ARO1-KMXK_A03020t PGK1pr-ARO2-LAC4t TEF1pr-ARO7fbr-PDC1t ENO1pr-PHA2-INU1t HSP150pr-ARO9-ScPGK1t This work
pI1-P8A9T1 pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t This work
pI1-P1A11T5 pI1-MTU-DO-HIS with insert PGK1pr-TAL1- KMXK_A03020t This work
pI1-PPP pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t TDH3pr-RPE1-PDC1t TSA1pr-RKI1-ScPGK1t This work
pI1-TKAL pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t This work
pI1-TKAL-ENO pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-KmENO1-ScADH1t This work
pI1-TKAL-A26 pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-CtPEPS-ScADH1t This work
pI1-TKAL-A27 pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-EcppsA-ScADH1t This work
pPPP-ENO pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-KmENO1-ScADH1t This work
pPPP-A26 pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-CtPEPS-ScADH1t This work
pPPP-A27 pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-EcppsA-ScADH1t This work
pPPP-A28 pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-TtPEPS-ScADH1t This work
pTKAL pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t This work
pP6B14T6-HIS pMTU-DO-HIS with insert HSP150pr-Caxfpk-PDC1t This work
pP6B17T6-HIS pMTU-DO-HIS with insert HSP150pr-Rgxfpk-PDC1t This work
pP6B18T6-HIS pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t This work
pP6B23T6-HIS pMTU-DO-HIS with insert HSP150pr-Llxfpk-PDC1t This work
pP16A10T3-HIS pMTU-DO-HIS with insert SSA2pr-KmENO1-ScADH1t This work
pP16A26T3-HIS pMTU-DO-HIS with insert SSA2pr-CtPEPS-ScADH1t This work
pP16A27T3-HIS pMTU-DO-HIS with insert SSA2pr-EcppsA-ScADH1t This work
pP16A28T3-HIS pMTU-DO-HIS with insert SSA2pr-TtPEPS-ScADH1t This work
pP16A29T3-HIS pMTU-DO-HIS with insert SSA2pr-AtPPDK-ScADH1t This work
pP16A37T3-HIS pMTU-DO-HIS with insert SSA2pr-AnPEPS-ScADH1t This work
pP16A39T3-HIS pMTU-DO-HIS with insert SSA2pr-HmPEPS-ScADH1t This work
pB18-A10 pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-KmENO1-ScADH1t This work
pB18-A27 pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-ppsA-ScADH1t This work
pB18-A28 pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-TtPEPS-ScADH1t This work
pI4-P6B18T6 pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t This work
pI4-pP16A10T3 pI4-MTU-DO-G418 with insert SSA2pr-KmENO1-ScADH1t This work
pI4-P16A26T3 pI4-MTU-DO-G418 with insert SSA2pr-CtPEPS-ScADH1t This work
pI4-P16A27T3 pI4-MTU-DO-G418 with insert SSA2pr-EcppsA-ScADH1t This work
pI4-P16A29T3 pI4-MTU-DO-G418 with insert SSA2pr-AtPPDK-ScADH1t This work
pI4-P16A37T3 pI4-MTU-DO-G418 with insert SSA2pr-AnPEPS-ScADH1t This work
pI4-P16A39T3 pI4-MTU-DO-G418 with insert SSA2pr-HmPEPS-ScADH1t This work
pI4-P20A30fT7 pI4-MTU-DO-G418 with insert FBA1pr-KmARO3fbr-PGK1t This work
pI1-B18-A27 pI1-MTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-EcppsA-ScADH1t This work
pI1-B38-HIS pI1-MTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t This work
pI4-B18-B15 pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-BsPta-KMXK_A03020t This work
pI4-B18-B20 pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t This work
pI4-B18-B15-A27 pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-BsPta-KMXK_A03020t SSA2pr-EcppsA-ScADH1t This work
pI4-B18-B20-A27 pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t SSA2pr-EcppsA-ScADH1t This work
pI2-B95-G418 pI2-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t FBApr-KmARO3fbr-PGK1t SSA2pr-ppsA-ScADH1t This work
pI2-B78-G418 pI2-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t FBApr-KmARO3fbr-PGK1t SSA2pr-KmENO1-ScADH1t Ā 
pI4-PEM pI4-MTU-DO-G418 with insert ENO1pr-ScARO10-INU1t TEF1pr-ScADH2-PGK1t This work
pA8-KO-P9 TYR1pr_US-REV1pr-TYR1pr_DS AmpR This work
pA8-KO-P11 TYR1pr_US-GDH2pr-TYR1pr_DS AmpR This work