Table 2 Expression plasmids used in this study. Unless specified otherwise, all genes, promoters and terminators in the inserts are from K. marxianus
Name | Contents/comments | Source |
|---|---|---|
pUCC001 | AaTEF1pr-SpCas9-ScPHO5t ScTDH3pr-HH-gRNAempty-HDV-ScCYC1t hphMX | [51] |
pMTU-DO-URA | Centromeric plasmid with GFP drop-out; ScURA3 KmARS/CEN7; available from Addgene (#160004) | This work |
pMTU-DO-HIS | Centromeric plasmid with GFP drop-out; ScHIS3 KmARS/CEN7; available from Addgene (#160005) | This work |
pI1-MTU-DO-HIS | Integrative plasmid with GFP drop-out targeting LAC4; ScHIS3; available from Addgene (#160142) | This work |
pI6-MTU-DO-URA | Integrative plasmid with GFP drop-out targeting ARO3; ScURA3; available from Addgene (#160030) | This work |
pI2-MTU-DO-G418 | Integrative plasmid with GFP drop-out, kanMX, targeting integration site I2 [27]; available from Addgene (#160018) | This work |
pI4-MTU-DO-G418 | Integrative plasmid with GFP drop-out, kanMX, targeting integration site I4 (Rajkumar et al., 2019); available from Addgene (#160215) | This work |
pA1f/A4f-U | pMTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TEF1pr-ARO7fbr-INU1t | This work |
pChor/A4f | pMTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TSA1pr-ARO1-KMXK_A03020t PGK1pr-ARO2-LAC4t TEF1pr-ARO7fbr-INU1t | This work |
pAAA/Phe | pMTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TSA1pr-ARO1-KMXK_A03020t PGK1pr-ARO2-LAC4t TEF1pr-ARO7fbr-PDC1t ENO1pr-PHA2-INU1t HSP150pr-ARO9-ScPGK1t | This work |
pI6-AAA/Phe | pI6-MTU-DO-URA with insert PDC1pr-ARO4fbr-ScADH1pr TSA1pr-ARO1-KMXK_A03020t PGK1pr-ARO2-LAC4t TEF1pr-ARO7fbr-PDC1t ENO1pr-PHA2-INU1t HSP150pr-ARO9-ScPGK1t | This work |
pI1-P8A9T1 | pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t | This work |
pI1-P1A11T5 | pI1-MTU-DO-HIS with insert PGK1pr-TAL1- KMXK_A03020t | This work |
pI1-PPP | pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t TDH3pr-RPE1-PDC1t TSA1pr-RKI1-ScPGK1t | This work |
pI1-TKAL | pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t | This work |
pI1-TKAL-ENO | pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-KmENO1-ScADH1t | This work |
pI1-TKAL-A26 | pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-CtPEPS-ScADH1t | This work |
pI1-TKAL-A27 | pI1-MTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-EcppsA-ScADH1t | This work |
pPPP-ENO | pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-KmENO1-ScADH1t | This work |
pPPP-A26 | pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-CtPEPS-ScADH1t | This work |
pPPP-A27 | pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-EcppsA-ScADH1t | This work |
pPPP-A28 | pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t SSA2pr-TtPEPS-ScADH1t | This work |
pTKAL | pMTU-DO-HIS with insert TEF1pr-TKL1-INU1t PGK1pr-TAL1- KMXK_A03020t | This work |
pP6B14T6-HIS | pMTU-DO-HIS with insert HSP150pr-Caxfpk-PDC1t | This work |
pP6B17T6-HIS | pMTU-DO-HIS with insert HSP150pr-Rgxfpk-PDC1t | This work |
pP6B18T6-HIS | pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t | This work |
pP6B23T6-HIS | pMTU-DO-HIS with insert HSP150pr-Llxfpk-PDC1t | This work |
pP16A10T3-HIS | pMTU-DO-HIS with insert SSA2pr-KmENO1-ScADH1t | This work |
pP16A26T3-HIS | pMTU-DO-HIS with insert SSA2pr-CtPEPS-ScADH1t | This work |
pP16A27T3-HIS | pMTU-DO-HIS with insert SSA2pr-EcppsA-ScADH1t | This work |
pP16A28T3-HIS | pMTU-DO-HIS with insert SSA2pr-TtPEPS-ScADH1t | This work |
pP16A29T3-HIS | pMTU-DO-HIS with insert SSA2pr-AtPPDK-ScADH1t | This work |
pP16A37T3-HIS | pMTU-DO-HIS with insert SSA2pr-AnPEPS-ScADH1t | This work |
pP16A39T3-HIS | pMTU-DO-HIS with insert SSA2pr-HmPEPS-ScADH1t | This work |
pB18-A10 | pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-KmENO1-ScADH1t | This work |
pB18-A27 | pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-ppsA-ScADH1t | This work |
pB18-A28 | pMTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-TtPEPS-ScADH1t | This work |
pI4-P6B18T6 | pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t | This work |
pI4-pP16A10T3 | pI4-MTU-DO-G418 with insert SSA2pr-KmENO1-ScADH1t | This work |
pI4-P16A26T3 | pI4-MTU-DO-G418 with insert SSA2pr-CtPEPS-ScADH1t | This work |
pI4-P16A27T3 | pI4-MTU-DO-G418 with insert SSA2pr-EcppsA-ScADH1t | This work |
pI4-P16A29T3 | pI4-MTU-DO-G418 with insert SSA2pr-AtPPDK-ScADH1t | This work |
pI4-P16A37T3 | pI4-MTU-DO-G418 with insert SSA2pr-AnPEPS-ScADH1t | This work |
pI4-P16A39T3 | pI4-MTU-DO-G418 with insert SSA2pr-HmPEPS-ScADH1t | This work |
pI4-P20A30fT7 | pI4-MTU-DO-G418 with insert FBA1pr-KmARO3fbr-PGK1t | This work |
pI1-B18-A27 | pI1-MTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t SSA2pr-EcppsA-ScADH1t | This work |
pI1-B38-HIS | pI1-MTU-DO-HIS with insert HSP150pr-Bbxfpk-PDC1t | This work |
pI4-B18-B15 | pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-BsPta-KMXK_A03020t | This work |
pI4-B18-B20 | pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t | This work |
pI4-B18-B15-A27 | pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-BsPta-KMXK_A03020t SSA2pr-EcppsA-ScADH1t | This work |
pI4-B18-B20-A27 | pI4-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t SSA2pr-EcppsA-ScADH1t | This work |
pI2-B95-G418 | pI2-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t FBApr-KmARO3fbr-PGK1t SSA2pr-ppsA-ScADH1t | This work |
pI2-B78-G418 | pI2-MTU-DO-G418 with insert HSP150pr-Bbxfpk-PDC1t TEF1pr-SePta-KMXK_A03020t FBApr-KmARO3fbr-PGK1t SSA2pr-KmENO1-ScADH1t | Â |
pI4-PEM | pI4-MTU-DO-G418 with insert ENO1pr-ScARO10-INU1t TEF1pr-ScADH2-PGK1t | This work |
pA8-KO-P9 | TYR1pr_US-REV1pr-TYR1pr_DS AmpR | This work |
pA8-KO-P11 | TYR1pr_US-GDH2pr-TYR1pr_DS AmpR | This work |