Table 1 Mutagenic primers used to carry out truncations in the α-MAT secretory signal
Primers | Primer Sequence |
|---|---|
Matα:WT | GCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAG |
Matα:Δ57–70 | F.P: 5′-GCTGTTTTGCCATTTTCCGCCAGCATTGCTGCTAAAG-3’ R.P: 5′-CTTTAGCAGCAATGCTGGCGGAAAATGGCAAAACAGC-3’ |
Matα:WT | CTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCG |
Matα:Δ30–43 | F.P: 5′-CTACAACAGAAGATGAATTAGAAGGGGATTTCG-3’ R.P: 5′-CGAAATCCCCTTCTAATTCATCTTCTGTTGTAG-3’ |
Matα:WT | CGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCC |
Matα:Δ47–49 | F.P: 5′-CGGTTACTCAGATTTAGAAGGGGTTGCTGTTTTGCCATTTTCC-3’ R.P: 5′-GGAAAATGGCAAAACAGCAACCCCTTCTAAATCTGAGTAACCG-3’ |