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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

Fig. 6

a SDS-PAGE analysis of extracellular G-CSF produced by transformants containing single deletions in the pro-secretory sequence fused to the CO-GCSF gene; Equal volume (10 μl) of the culture filtrate was loaded after 120 h of cultivation in a 500 ml Erlenmeyer flask. Lane 1: Molecular weight ladder; Lane 2; empty vector; Lane 3: Matα:WT fused to the CO-GCSF gene; Lanes 4 & 5: Matα:Δ57–70, Cl #s 25, 32; Lanes 6 & 7: Matα:Δ30–43, Cl# 12, 32; Lane 8: Filgrastim (1.5 μg); Lanes 9 & 10: Matα:Δ47–49, Cl #s 44, 45. b SDS-PAGE analysis of the G-CSF produced by transformants containing double deletions in the pro-secretory sequence fused to the CO-GCSF gene; Lane 11: Molecular weight ladder; Lane 12; Filgrastim (1.5 μg); Lane 13: Matα:WT fused to the CO-GCSF gene; Lanes 14 &15: Matα:Δ57–70, Cl# 25, 32; Lanes 16 &17: Matα:Δ57–70;47–49, Cl# 17,18; Lanes 18 & 19: Matα:Δ57–70;30–43, Cl# 23, 33. c Cell O.D, total extracellular protein and G-CSF titre at 120 h post-methanol induction. The units for cell O.D. are the same as shown on the Y-axis

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Microbial Cell Factories

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